Fluorescent probes for
reactive species detection.
Aminophenyl- and nitrophenyl-fluorescein derivatives for selective, cell-permeable detection of highly reactive oxygen and nitrogen species (hROS and hRNS) in live-cell and biochemical assay systems.
APF is a cell-permeable and photostable fluorescent probe designed for the selective detection of highly reactive oxygen and nitrogen species (hROS and hRNS). Upon reaction with hROS/hRNS, APF is cleaved to release free fluorescein, generating a strong fluorescence signal detectable by standard fluorescence microscopy, flow cytometry and plate reader systems.
- Live-cell imaging of oxidative burst and nitrosative stress
- Flow cytometry quantification of hROS/hRNS production
- High-content screening for antioxidant compounds
- Mechanistic studies of NF-κB, apoptosis, and inflammation pathways
- Antimicrobial and phagocytosis assay systems
Nitrophenyl fluorescein serves as a substrate or reporter group in enzymatic and bioanalytical assay systems. A chemical transformation — typically enzyme-mediated hydrolysis or reduction of the nitrophenyl ester bond — changes the compound's absorbance and fluorescence properties, generating a measurable optical signal proportional to enzyme activity or analyte concentration.
- Enzymatic activity assays — esterases, lipases, hydrolases
- Bioanalytical detection of specific analytes via reporter release
- High-throughput screening substrates for enzyme panels
- Quality control and calibration standards in optical assays
- Custom reporter substrate design under NDA
For research groups and industrial labs working on oxidative stress biology, enzyme activity profiling, or antioxidant screening — we offer APF and NPF as a combined probe panel. The complementary selectivity profiles of the two compounds allow discrimination between hROS/hRNS-mediated effects (APF) and enzyme-catalyzed transformations (NPF) within the same experimental system.
Synthetic compounds designed to mimic the structure and function of natural sphingolipids. The pyrrolidine ring (five-membered nitrogen heterocycle) enhances chemical stability and affinity for specific enzymes. Key candidates for enzyme inhibitor development and lysosomal disorder research.
Natural components found in plants, yeasts and human skin. Four stereoisomers (D/L-ribo and D/L-arabino) differ in spatial arrangement of hydroxyl groups — enabling enzyme stereoselectivity studies. D-ribo-phytosphingosine is the most common natural form.
Dihydrosphingosine derivatives where the C3 hydroxyl is replaced by an amino group (–NH₂). D-erythro and L-erythro isomers show distinct biological activity. The amino group enables stronger protein kinase C (PKC) inhibition — relevant for cell signalling and apoptosis research.
Sphingosine is the fundamental building block of sphingolipids. Our analogues feature modified chain lengths or functional groups for precise modulation of sphingosine-1-phosphate (S1P) signalling pathways — important in autoimmune disease therapy (multiple sclerosis) and tumour angiogenesis control.
Sugar isothiocyanates are highly reactive intermediates capable of forming covalent bonds with amines — used for bioconjugation of sugars to proteins or fluorescent labels, enabling sugar transport tracking in vivo. Aminosugars (–OH replaced by –NH₂) are key building blocks in glycoprotein and glycolipid synthesis, with applications in joint nutrition (chondroprotectives) and novel antibiotic development targeting bacterial cell walls.